Immune monitoring often entails measuring responses of B and T cells to antigens. The most common method for monitoring lymphocyte responses is the ELISPOT (Enzyme-Linked ImmunoSpot) Assay. The ELISPOT provides a single-cell secretory footprint in the form of a colored spot that is scanned and counted for statistical significance.
An MHC tetramer assay or simply tetramer assay is a procedure of use labeled tetrameric protein to detect and quantify T-cells that are specific for a given antigen within blood or tissue samples. The IML can produce both MHC class I and class II tetramers for use in both mouse and human systems, which can be adapted for detection by either flow cytometry or CyTOF.