Mass Cytometry CyTOF2 Helios

Mass Cytometry CyTOF2 Helios

The IML is equipped with two (2) state-of-the-art CyTOF2/Helios (Fluidigm) mass cytometers.

CyTOF2 and Helios analyze cells labeled with a panel of reagents conjugated to stable metal isotopes using cutting-edge time-of-flight mass cytometry technology.

Mass cytometry resolves multiple metal probes per cell with minimal signal overlap, maximizing the information obtained from a single sample. With over 120 available detection channels and over 40 isotopic probes, mass cytometry enables comprehensive profiling of cellular phenotype, signaling state, cytokine/chemokine expression, and viability.

Useful Links 

Fluidigm Catalog

  • a catalog of metal-conjugated antibodies and other reagents available from Fluidigm, Inc. 

Fluidigm Panel Designer Tool

Cytoforum 

  • a discussion board for CyTOF related questions

CyTOF mini-course

  • a collection of YouTube videos from an annual CyTOF mini-course organized by Garry Nolan Lab. The Nolan Lab page contains other useful resources as well.

Cytobank Webinars on YouTube