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Equipment & Services
The Immunomonitoring Lab provides testing & tissue processing services to its participating members. Below is a list of equipment that is utilized within our core lab along with hyperlinks to additional information on the machine and/or process.
Blood Processing
PBMC Isolation and Plasma Collection from Leukopaks and Vacutainers
Tetramer Assays
An MHC tetramer assay or simply tetramer assay is a procedure of use labeled tetrameric protein to detect and quantify T-cells that are specific for a given antigen within blood or tissue samples. The IML can produce both MHC class I and class II tetramers for use in both mouse and human systems, which can be adapted for detection by either flow cytometry or CyTOF.
BioTek Synergy H1 Plate Reader
Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader.
CTL-Immunospot S6 Universal Analyzer (ELISPOT)
Immune monitoring often entails measuring responses of B and T cells to antigens. The most common method for monitoring lymphocyte responses is the ELISPOT (Enzyme-Linked ImmunoSpot) Assay. The ELISPOT provides a single-cell secretory footprint in the form of a colored spot that is scanned and counted for statistical significance.
Luminex FLEXMAP 3D
The Luminex platform is capable of multi-analyte screening of samples using flow cytometry to detect analytes captured on polystyrene or magnetic beads.
Flow Cytometry Fortessa X20 Cytometer
The BD LSRFortessa X-20 flow cytometry analyzer is configured with 5 lasers and is able to detect up to 20 parameters simultaneously.
Mass Cytometry CyTOF2 Helios
The IML is equipped with two (2) state-of-the-art CyTOF2/Helios (Standard BioTools, Inc. – FKA Fluidigm) mass cytometers.