Equipment & Services

The Immunomonitoring Lab provides testing & tissue processing services to its participating members.  Below is a list of equipment that is utilized within our core lab along with hyperlinks to additional information on the machine and/or process.

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Blood Processing

Blood Processing

PBMC Isolation and Plasma Collection from Leukopaks and Vacutainers

Tetramer Assays

Tetramer Assays

An MHC tetramer assay or simply tetramer assay is a procedure of use labeled tetrameric protein to detect and quantify T-cells that are specific for a given antigen within blood or tissue samples. The IML can produce both MHC class I and class II tetramers for use in both mouse and human systems, which can be adapted for detection by either flow cytometry or CyTOF.

BioTek Synergy H1 Plate Reader

BioTek Synergy H1 Plate Reader

Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader.

CTL-Immunospot S6 Universal Analyzer (ELISPOT)

CTL-Immunospot S6 Universal Analyzer (ELISPOT)

Immune monitoring often entails measuring responses of B and T cells to antigens.  The most common method for monitoring lymphocyte responses is the ELISPOT (Enzyme-Linked ImmunoSpot) Assay.  The ELISPOT provides a single-cell secretory footprint in the form of a colored spot that is scanned and counted for statistical significance.

Luminex FLEXMAP 3D

Luminex FLEXMAP 3D

The Luminex platform is capable of multi-analyte screening of samples using flow cytometry to detect analytes captured on polystyrene or magnetic beads.

Flow Cytometry Fortessa X20 Cytometer

Flow Cytometry Fortessa X20 Cytometer

The BD LSRFortessa X-20 flow cytometry analyzer is configured with 5 lasers and is able to detect up to 20 parameters simultaneously.

Mass Cytometry CyTOF2 Helios

Mass Cytometry CyTOF2 Helios

The IML is equipped with two (2) state-of-the-art CyTOF2/Helios (Standard BioTools, Inc. – FKA Fluidigm) mass cytometers.